REFERENCE STANDARD AND WORKING STANDARD !!

 ! REFERENCE STANDARD AND WORKING STANDARD !!

Reference standards and working standards are terms commonly used in analytical chemistry. They serve different purposes in the calibration and validation of analytical methods.

👑 Reference Standard:

🌲 Definition: A reference standard is a highly pure substance with a known and well-defined concentration or potency. It is typically used as a benchmark or reference point in analytical measurements.

🌲 Characteristics:

🔹 The reference standard is of the highest available purity and quality.

🔹Its concentration or potency is precisely determined through reliable analytical techniques, and it serves as a reference for comparison with other samples.

🔹Reference standards are often used to calibrate instruments, validate methods and ensure the accuracy of measurements.

🔹Examples include certified reference materials (CRM) or primary standards.

🔹Reference standards are usually obtained from a recognized source, such as a pharmacopeia and are used to verify the accuracy of working standards. 

🔹The use of reference standards ensures that the manufactured product meets the standards set by regulatory authorities and the pharmacopeia.

👑Working Standard:

🌲Definition: A working standard is a substance with a known concentration or potency that is used routinely in the laboratory for calibration, quality control, and day-to-day analytical work.

🌲Characteristics:

🔹Working standards are prepared in-house from reference standards but may have a slightly lower level of precision and accuracy compared to reference standards.

🔹They are used for routine calibration of instruments, validation of methods, and quality control checks during the analysis of samples.

🔹Working standards are more accessible and are consumed in regular laboratory operations.

🔹Examples include in-house prepared solutions or materials that are periodically calibrated against reference standards.